Demersal fish diversity and molecular taxonomy in the Bering Sea and Chukchi Sea

DNA barcoding by sequencing a standard region of cytochrome c oxidase subunit I (COI) provides an accurate, rapid method for identifying different species. In this study, we provide molecular taxonomic assessment demersal fishes in the Bering Sea and Chukchi based on barcoding, total 123 mitochondrial COI partial fragments with length 652 bp were obtained. The consensus among all sequences was determined alignment via BLAST search GenBank. Phylogenetic relationships reconstructed neighbor-joining trees gaps. 39 species investigated analysis distributed 10 families. Five families within Scorpaeniformes including 19 accounted almost half next largest group Perciformes, 9 species, followed Pleuronectiformes Gadiformes, 5 each, smallest number belonged to Rajiformes. At family level, Cottidae family, Zoarcidae, accounting 8 other eight families—Gadidae, Pleuronectidae, Psychrolutidae, Agonidae, Liparidae, Ammodytidae, Hexagrammidae, Rajidae—accounted smaller proportion brief, our study shows that barcodes are effective tool studying fish diversity taxonomy Sea. contribution Arctic may benefit further studies biodiversity, biogeography conservation future.